MersV1, Main, Exploration, bibRecord, 001331

Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR

Identifieur interne : 001331 ( Main/Exploration ); précédent : 001330; suivant : 001332

Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR

Auteurs : Heungsup Sung [Corée du Sud] ; Dongeun Yong [Corée du Sud] ; Chang-Seok Ki [Corée du Sud] ; Jae-Seok Kim [Corée du Sud] ; Moon-Woo Seong [Corée du Sud] ; Hyukmin Lee [Corée du Sud] ; Mi-Na Kim [Corée du Sud]

Source :

Annals of Laboratory Medicine [ 2234-3806 ] ; 2016.

RBID : PMC:4940489

Descripteurs français

KwdFr :
- ARN viral (analyse), ARN viral (isolement et purification), ARN viral (métabolisme), Acétylcystéine (), Citrates (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Deoxyribonuclease I (métabolisme), Endopeptidase K (métabolisme), Expectoration (virologie), Humains, Infections à coronavirus (diagnostic), Réaction de polymérisation en chaine en temps réel.
MESH :
- analyse : ARN viral.
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : ARN viral, Coronavirus du syndrome respiratoire du Moyen-Orient.
- métabolisme : ARN viral, Deoxyribonuclease I, Endopeptidase K.
- virologie : Expectoration.
- Acétylcystéine, Citrates, Humains, Réaction de polymérisation en chaine en temps réel.

English descriptors

KwdEn :
- Acetylcysteine (chemistry), Citrates (chemistry), Coronavirus Infections (diagnosis), Deoxyribonuclease I (metabolism), Endopeptidase K (metabolism), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), RNA, Viral (analysis), RNA, Viral (isolation & purification), RNA, Viral (metabolism), Real-Time Polymerase Chain Reaction, Sodium Citrate, Sputum (virology).
MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : Acetylcysteine, Citrates.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus, RNA, Viral.
- chemical , metabolism : Deoxyribonuclease I, Endopeptidase K, RNA, Viral.
- virology : Sputum.
- Humans, Real-Time Polymerase Chain Reaction, Sodium Citrate.

Abstract

Background

Real-time reverse transcription PCR (rRT-PCR) of sputum samples is commonly used to diagnose Middle East respiratory syndrome coronavirus (MERS-CoV) infection. Owing to the difficulty of extracting RNA from sputum containing mucus, sputum homogenization is desirable prior to nucleic acid isolation. We determined optimal homogenization methods for isolating viral nucleic acids from sputum.

Methods

We evaluated the following three sputum-homogenization methods: proteinase K and DNase I (PK-DNase) treatment, phosphate-buffered saline (PBS) treatment, and N-acetyl-L-cysteine and sodium citrate (NALC) treatment. Sputum samples were spiked with inactivated MERS-CoV culture isolates. RNA was extracted from pretreated, spiked samples using the easyMAG system (bioMérieux, France). Extracted RNAs were then subjected to rRT-PCR for MERS-CoV diagnosis (DiaPlex Q MERS-coronavirus, SolGent, Korea).

Results

While analyzing 15 spiked sputum samples prepared in technical duplicate, false-negative results were obtained with five (16.7%) and four samples (13.3%), respectively, by using the PBS and NALC methods. The range of threshold cycle (Ct) values observed when detecting upE in sputum samples was 31.1–35.4 with the PK-DNase method, 34.7–39.0 with the PBS method, and 33.9–38.6 with the NALC method. Compared with the control, which were prepared by adding a one-tenth volume of 1:1,000 diluted viral culture to PBS solution, the ranges of Ct values obtained by the PBS and NALC methods differed significantly from the mean control Ct of 33.2 (both P<0.0001).

Conclusions

The PK-DNase method is suitable for homogenizing sputum samples prior to RNA extraction.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940489

DOI: 10.3343/alm.2016.36.5.457
PubMed: 27374711
PubMed Central: 4940489

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR</title>
<author><name sortKey="Sung, Heungsup" sort="Sung, Heungsup" uniqKey="Sung H" first="Heungsup" last="Sung">Heungsup Sung</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yong, Dongeun" sort="Yong, Dongeun" uniqKey="Yong D" first="Dongeun" last="Yong">Dongeun Yong</name>
<affiliation wicri:level="3"><nlm:aff id="A2">Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ki, Chang Seok" sort="Ki, Chang Seok" uniqKey="Ki C" first="Chang-Seok" last="Ki">Chang-Seok Ki</name>
<affiliation wicri:level="3"><nlm:aff id="A3">Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Jae Seok" sort="Kim, Jae Seok" uniqKey="Kim J" first="Jae-Seok" last="Kim">Jae-Seok Kim</name>
<affiliation wicri:level="3"><nlm:aff id="A4">Department of Laboratory Medicine, Hallym University College of Medicine, Hallym University Kangdong Sacred Heart Hospital, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Hallym University College of Medicine, Hallym University Kangdong Sacred Heart Hospital, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon-Woo" last="Seong">Moon-Woo Seong</name>
<affiliation wicri:level="3"><nlm:aff id="A5">Department of Laboratory Medicine, Seoul National University College of Medicine and Seoul National University Hospital, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Seoul National University College of Medicine and Seoul National University Hospital, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Hyukmin" sort="Lee, Hyukmin" uniqKey="Lee H" first="Hyukmin" last="Lee">Hyukmin Lee</name>
<affiliation wicri:level="1"><nlm:aff id="A6">Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon</wicri:regionArea>
<wicri:noRegion>Incheon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Mi Na" sort="Kim, Mi Na" uniqKey="Kim M" first="Mi-Na" last="Kim">Mi-Na Kim</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27374711</idno>
<idno type="pmc">4940489</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4940489</idno>
<idno type="RBID">PMC:4940489</idno>
<idno type="doi">10.3343/alm.2016.36.5.457</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000081</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000081</idno>
<idno type="wicri:Area/Pmc/Curation">000081</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000081</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000C19</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000C19</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:27374711</idno>
<idno type="wicri:Area/PubMed/Corpus">001059</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001059</idno>
<idno type="wicri:Area/PubMed/Curation">001059</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001059</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001177</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001177</idno>
<idno type="wicri:Area/Ncbi/Merge">001685</idno>
<idno type="wicri:Area/Ncbi/Curation">001685</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001685</idno>
<idno type="wicri:doubleKey">2234-3806:2016:Sung H:comparative:evaluation:of</idno>
<idno type="wicri:Area/Main/Merge">001335</idno>
<idno type="wicri:Area/Main/Curation">001331</idno>
<idno type="wicri:Area/Main/Exploration">001331</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR</title>
<author><name sortKey="Sung, Heungsup" sort="Sung, Heungsup" uniqKey="Sung H" first="Heungsup" last="Sung">Heungsup Sung</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yong, Dongeun" sort="Yong, Dongeun" uniqKey="Yong D" first="Dongeun" last="Yong">Dongeun Yong</name>
<affiliation wicri:level="3"><nlm:aff id="A2">Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ki, Chang Seok" sort="Ki, Chang Seok" uniqKey="Ki C" first="Chang-Seok" last="Ki">Chang-Seok Ki</name>
<affiliation wicri:level="3"><nlm:aff id="A3">Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kim, Jae Seok" sort="Kim, Jae Seok" uniqKey="Kim J" first="Jae-Seok" last="Kim">Jae-Seok Kim</name>
<affiliation wicri:level="3"><nlm:aff id="A4">Department of Laboratory Medicine, Hallym University College of Medicine, Hallym University Kangdong Sacred Heart Hospital, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Hallym University College of Medicine, Hallym University Kangdong Sacred Heart Hospital, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon-Woo" last="Seong">Moon-Woo Seong</name>
<affiliation wicri:level="3"><nlm:aff id="A5">Department of Laboratory Medicine, Seoul National University College of Medicine and Seoul National University Hospital, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, Seoul National University College of Medicine and Seoul National University Hospital, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lee, Hyukmin" sort="Lee, Hyukmin" uniqKey="Lee H" first="Hyukmin" last="Lee">Hyukmin Lee</name>
<affiliation wicri:level="1"><nlm:aff id="A6">Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon</wicri:regionArea>
<wicri:noRegion>Incheon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kim, Mi Na" sort="Kim, Mi Na" uniqKey="Kim M" first="Mi-Na" last="Kim">Mi-Na Kim</name>
<affiliation wicri:level="3"><nlm:aff id="A1">Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, Korea.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Corée du Sud</country>
<wicri:regionArea>Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
<region type="capital">Région capitale de Séoul</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Annals of Laboratory Medicine</title>
<idno type="ISSN">2234-3806</idno>
<idno type="eISSN">2234-3814</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acetylcysteine (chemistry)</term>
<term>Citrates (chemistry)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Deoxyribonuclease I (metabolism)</term>
<term>Endopeptidase K (metabolism)</term>
<term>Humans</term>
<term>Middle East Respiratory Syndrome Coronavirus (genetics)</term>
<term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term>
<term>RNA, Viral (analysis)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>RNA, Viral (metabolism)</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sodium Citrate</term>
<term>Sputum (virology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>ARN viral (isolement et purification)</term>
<term>ARN viral (métabolisme)</term>
<term>Acétylcystéine ()</term>
<term>Citrates ()</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term>
<term>Deoxyribonuclease I (métabolisme)</term>
<term>Endopeptidase K (métabolisme)</term>
<term>Expectoration (virologie)</term>
<term>Humains</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acetylcysteine</term>
<term>Citrates</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Deoxyribonuclease I</term>
<term>Endopeptidase K</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term>
<term>Deoxyribonuclease I</term>
<term>Endopeptidase K</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Expectoration</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Sputum</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sodium Citrate</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Acétylcystéine</term>
<term>Citrates</term>
<term>Humains</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Real-time reverse transcription PCR (rRT-PCR) of sputum samples is commonly used to diagnose Middle East respiratory syndrome coronavirus (MERS-CoV) infection. Owing to the difficulty of extracting RNA from sputum containing mucus, sputum homogenization is desirable prior to nucleic acid isolation. We determined optimal homogenization methods for isolating viral nucleic acids from sputum.</p>
</sec>
<sec><title>Methods</title>
<p>We evaluated the following three sputum-homogenization methods: proteinase K and DNase I (PK-DNase) treatment, phosphate-buffered saline (PBS) treatment, and <italic>N</italic>
-acetyl-L-cysteine and sodium citrate (NALC) treatment. Sputum samples were spiked with inactivated MERS-CoV culture isolates. RNA was extracted from pretreated, spiked samples using the easyMAG system (bioMérieux, France). Extracted RNAs were then subjected to rRT-PCR for MERS-CoV diagnosis (DiaPlex Q MERS-coronavirus, SolGent, Korea).</p>
</sec>
<sec><title>Results</title>
<p>While analyzing 15 spiked sputum samples prepared in technical duplicate, false-negative results were obtained with five (16.7%) and four samples (13.3%), respectively, by using the PBS and NALC methods. The range of threshold cycle (Ct) values observed when detecting <italic>upE</italic>
 in sputum samples was 31.1–35.4 with the PK-DNase method, 34.7–39.0 with the PBS method, and 33.9–38.6 with the NALC method. Compared with the control, which were prepared by adding a one-tenth volume of 1:1,000 diluted viral culture to PBS solution, the ranges of Ct values obtained by the PBS and NALC methods differed significantly from the mean control Ct of 33.2 (both <italic>P</italic>
<0.0001).</p>
</sec>
<sec><title>Conclusions</title>
<p>The PK-DNase method is suitable for homogenizing sputum samples prior to RNA extraction.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lee, Jh" uniqKey="Lee J">JH Lee</name>
</author>
<author><name sortKey="Lee, Cs" uniqKey="Lee C">CS Lee</name>
</author>
<author><name sortKey="Lee, Hb" uniqKey="Lee H">HB Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wilson, D" uniqKey="Wilson D">D Wilson</name>
</author>
<author><name sortKey="Yen Lieberman, B" uniqKey="Yen Lieberman B">B Yen-Lieberman</name>
</author>
<author><name sortKey="Reischl, U" uniqKey="Reischl U">U Reischl</name>
</author>
<author><name sortKey="Warshawsky, I" uniqKey="Warshawsky I">I Warshawsky</name>
</author>
<author><name sortKey="Procop, Gw" uniqKey="Procop G">GW Procop</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B Guery</name>
</author>
<author><name sortKey="Poissy, J" uniqKey="Poissy J">J Poissy</name>
</author>
<author><name sortKey="El Mansouf, L" uniqKey="El Mansouf L">L el Mansouf</name>
</author>
<author><name sortKey="Sejourne, C" uniqKey="Sejourne C">C Séjourné</name>
</author>
<author><name sortKey="Ettahar, N" uniqKey="Ettahar N">N Ettahar</name>
</author>
<author><name sortKey="Lemaire, X" uniqKey="Lemaire X">X Lemaire</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kapoor, M" uniqKey="Kapoor M">M Kapoor</name>
</author>
<author><name sortKey="Pringle, K" uniqKey="Pringle K">K Pringle</name>
</author>
<author><name sortKey="Kumar, A" uniqKey="Kumar A">A Kumar</name>
</author>
<author><name sortKey="Dearth, S" uniqKey="Dearth S">S Dearth</name>
</author>
<author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name>
</author>
<author><name sortKey="Lovchik, J" uniqKey="Lovchik J">J Lovchik</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name>
</author>
<author><name sortKey="Seilmaier, M" uniqKey="Seilmaier M">M Seilmaier</name>
</author>
<author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name>
</author>
<author><name sortKey="Hartmann, W" uniqKey="Hartmann W">W Hartmann</name>
</author>
<author><name sortKey="Scheible, G" uniqKey="Scheible G">G Scheible</name>
</author>
<author><name sortKey="Sack, S" uniqKey="Sack S">S Sack</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name>
</author>
<author><name sortKey="Al Tawfiq, Ja" uniqKey="Al Tawfiq J">JA Al-Tawfiq</name>
</author>
<author><name sortKey="Makhdoom, Hq" uniqKey="Makhdoom H">HQ Makhdoom</name>
</author>
<author><name sortKey="Assiri, A" uniqKey="Assiri A">A Assiri</name>
</author>
<author><name sortKey="Alhakeem, Rf" uniqKey="Alhakeem R">RF Alhakeem</name>
</author>
<author><name sortKey="Albarrak, A" uniqKey="Albarrak A">A Albarrak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Murray, Pr" uniqKey="Murray P">PR Murray</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kim, Hk" uniqKey="Kim H">HK Kim</name>
</author>
<author><name sortKey="Oh, Sh" uniqKey="Oh S">SH Oh</name>
</author>
<author><name sortKey="Yun, Ka" uniqKey="Yun K">KA Yun</name>
</author>
<author><name sortKey="Sung, H" uniqKey="Sung H">H Sung</name>
</author>
<author><name sortKey="Kim, Mn" uniqKey="Kim M">MN Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sweeney, Pj" uniqKey="Sweeney P">PJ Sweeney</name>
</author>
<author><name sortKey="Walker, Jm" uniqKey="Walker J">JM Walker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Breitbart, B" uniqKey="Breitbart B">B Breitbart</name>
</author>
<author><name sortKey="Rohwer, F" uniqKey="Rohwer F">F Rohwer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Desjardin, Le" uniqKey="Desjardin L">LE Desjardin</name>
</author>
<author><name sortKey="Perkins, Md" uniqKey="Perkins M">MD Perkins</name>
</author>
<author><name sortKey="Teixeira, L" uniqKey="Teixeira L">L Teixeira</name>
</author>
<author><name sortKey="Cave, Md" uniqKey="Cave M">MD Cave</name>
</author>
<author><name sortKey="Eisenach, Kd" uniqKey="Eisenach K">KD Eisenach</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Erin, Em" uniqKey="Erin E">EM Erin</name>
</author>
<author><name sortKey="Jenkins, Gr" uniqKey="Jenkins G">GR Jenkins</name>
</author>
<author><name sortKey="Kon, Om" uniqKey="Kon O">OM Kon</name>
</author>
<author><name sortKey="Zacharasiewicz, As" uniqKey="Zacharasiewicz A">AS Zacharasiewicz</name>
</author>
<author><name sortKey="Nicholson, Gc" uniqKey="Nicholson G">GC Nicholson</name>
</author>
<author><name sortKey="Neighbour, H" uniqKey="Neighbour H">H Neighbour</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Grebski, E" uniqKey="Grebski E">E Grebski</name>
</author>
<author><name sortKey="Peterson, C" uniqKey="Peterson C">C Peterson</name>
</author>
<author><name sortKey="Medici, Tc" uniqKey="Medici T">TC Medici</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zumla, A" uniqKey="Zumla A">A Zumla</name>
</author>
<author><name sortKey="Hui, Ds" uniqKey="Hui D">DS Hui</name>
</author>
<author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A Assiri</name>
</author>
<author><name sortKey="Al Tawfiq, Ja" uniqKey="Al Tawfiq J">JA Al-Tawfiq</name>
</author>
<author><name sortKey="Al Rabeeah, Aa" uniqKey="Al Rabeeah A">AA Al-Rabeeah</name>
</author>
<author><name sortKey="Al Rabiah, Fa" uniqKey="Al Rabiah F">FA Al-Rabiah</name>
</author>
<author><name sortKey="Al Hajjar, S" uniqKey="Al Hajjar S">S Al-Hajjar</name>
</author>
<author><name sortKey="Al Barrak, A" uniqKey="Al Barrak A">A Al-Barrak</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name>
</author>
<author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name>
</author>
<author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name>
</author>
<author><name sortKey="Dekkers, Dh" uniqKey="Dekkers D">DH Dekkers</name>
</author>
<author><name sortKey="Mller, Ma" uniqKey="Mller M">MA Mller</name>
</author>
<author><name sortKey="Dijkman, R" uniqKey="Dijkman R">R Dijkman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poissy, J" uniqKey="Poissy J">J Poissy</name>
</author>
<author><name sortKey="Goffard, A" uniqKey="Goffard A">A Goffard</name>
</author>
<author><name sortKey="Parmentier Decrucq, E" uniqKey="Parmentier Decrucq E">E Parmentier-Decrucq</name>
</author>
<author><name sortKey="Favory, R" uniqKey="Favory R">R Favory</name>
</author>
<author><name sortKey="Kauv, M" uniqKey="Kauv M">M Kauv</name>
</author>
<author><name sortKey="Kipnis, E" uniqKey="Kipnis E">E Kipnis</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Corée du Sud</li>
</country>
<region><li>Région capitale de Séoul</li>
</region>
<settlement><li>Séoul</li>
</settlement>
</list>
<tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Sung, Heungsup" sort="Sung, Heungsup" uniqKey="Sung H" first="Heungsup" last="Sung">Heungsup Sung</name>
</region>
<name sortKey="Ki, Chang Seok" sort="Ki, Chang Seok" uniqKey="Ki C" first="Chang-Seok" last="Ki">Chang-Seok Ki</name>
<name sortKey="Kim, Jae Seok" sort="Kim, Jae Seok" uniqKey="Kim J" first="Jae-Seok" last="Kim">Jae-Seok Kim</name>
<name sortKey="Kim, Mi Na" sort="Kim, Mi Na" uniqKey="Kim M" first="Mi-Na" last="Kim">Mi-Na Kim</name>
<name sortKey="Lee, Hyukmin" sort="Lee, Hyukmin" uniqKey="Lee H" first="Hyukmin" last="Lee">Hyukmin Lee</name>
<name sortKey="Seong, Moon Woo" sort="Seong, Moon Woo" uniqKey="Seong M" first="Moon-Woo" last="Seong">Moon-Woo Seong</name>
<name sortKey="Yong, Dongeun" sort="Yong, Dongeun" uniqKey="Yong D" first="Dongeun" last="Yong">Dongeun Yong</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001331 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001331 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4940489
   |texte=   Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:27374711" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a MersV1

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

Serveur d'exploration MERS

Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR

Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.